Mapping of Subunit-Subunit Contact Surfaces on the β′ Subunit of Escherichia coli RNA Polymerase
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چکیده
منابع مشابه
Determinants for Escherichia coli RNA Polymerase Assembly within the β Subunit
0022±2836/97/300648±15 $25.00/0/mb We used binding assays and other approaches to identify fragments of the Escherichia coli RNAP b subunit involved in the obligatory interaction with the a subunit to form the stable assembly intermediate a2b as well as in the interaction to recruit the b0 subunit into the a2b sub-assembly. We show that two regions of evolutionarily conserved sequence near the ...
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The function of the subunit of Escherichia coli DNA polymerase III holoenzyme is not well established. is a tightly bound component of the DNA polymerase III core, which contains the subunit (polymerase), the subunit (3 35 exonuclease), and the subunit, in the linear order . Previous studies have shown that the subunit is not essential, as strains carrying a deletion of the holE gene (which enc...
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Mapping of protein positions in the ribosomal subunits was first achieved for the 30S subunit by means of neutron scattering about 15 years ago. Since the 50S subunit is almost twice as large as the 30S subunit and consists of more proteins, it was difficult to apply classical contrast variation techniques for the localisation of the proteins. Polarisation dependent neutron scattering (spin-con...
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Transcription of the ferric citrate transport system of Escherichia coli K-12 is mediated by the extracytoplasmic-function (ECF) sigma factor FecI, which is activated by ferric citrate in the growth medium. By using a bacterial two-hybrid system, it was shown in vivo that FecI binds to the subunit of RNA polymerase. The inactive mutant protein FecI(K155E) displayed reduced binding to , and smal...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.5.3583